Detection specificity of the platform. Credit score: Microsystems & Nanoengineering (2024). DOI: 10.1038/s41378-024-00822-1
A workforce of researchers from the College of Florida has developed an revolutionary handheld system for human immunodeficiency virus (HIV) detection that mixes paper-based pattern preparation with real-time isothermal amplification. This low-cost, transportable platform guarantees fast and correct HIV analysis on the level of care (POC), overcoming the restrictions of conventional laboratory-based checks.
By eliminating the necessity for advanced tools and specialised personnel, this system is poised to revolutionize early HIV detection and administration, notably in resource-limited settings.
With outcomes delivered in as little as 60 minutes, the system gives unprecedented pace and reliability, matching the sensitivity of ordinary laboratory assays.
Human immunodeficiency virus (HIV) stays a world well being problem, with hundreds of thousands of individuals affected worldwide. Early analysis is important for well timed antiretroviral remedy and stopping transmission. Nonetheless, present nucleic acid checks (NATs) usually require superior laboratory infrastructure and skilled personnel, making them impractical in low-resource settings.
Whereas antibody-based fast checks present comfort, they lack sensitivity in the course of the acute part of an infection and fail to tell apart between acute and power phases. These limitations spotlight the pressing want for reasonably priced, delicate, and transportable HIV detection instruments that may be simply deployed on the level of care (POC).
In September 2024, a workforce from the College of Florida unveiled their handheld HIV detection platform in Microsystems & Nanoengineering.
This system integrates paper-based pattern preparation with real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP), providing an reasonably priced and environment friendly various for HIV testing. The system stands as a breakthrough in POC diagnostics, combining simplicity, portability, and excessive sensitivity to create an answer that might rework international HIV testing.
The brand new HIV detection platform gives a novel integration of paper-based pattern preparation and isothermal amplification, eliminating the necessity for lab tools like centrifuges or pipettes. Viral RNA is extracted from samples onto a paper substrate, and subsequently amplified through RT-LAMP in a compact, battery-powered heating unit.
Detection is achieved by means of colorimetric indicators or real-time imaging. The system demonstrated a detection restrict of 30 copies/mL of HIV RNA, a stage similar to conventional laboratory strategies, with no cross-reactivity to hepatitis C virus (HCV). Medical testing confirmed 100% specificity, guaranteeing the system’s accuracy for HIV analysis.
Furthermore, the real-time detection functionality permits for quantitative viral load measurement, which is vital for assessing remedy efficacy and drug resistance.
By delivering ends in underneath 60 minutes, the system considerably reduces the time required in comparison with conventional PCR-based strategies, offering a fast diagnostic answer in resource-poor environments.
Dr. Z. Hugh Fan, a key researcher on the venture, stated, “Our handheld device addresses the critical need for rapid, accurate, and accessible HIV testing. By integrating sample preparation and real-time detection in a portable format, we have created a tool that can change the landscape of HIV diagnosis, particularly in resource-limited areas where laboratory infrastructure is scarce.”
This handheld HIV detection platform can have wide-reaching implications for international well being. Its low price, portability, and excessive sensitivity make it a super device for POC testing in low-resource environments, the place entry to conventional diagnostic strategies is restricted.
Along with bettering HIV administration and remedy outcomes, the expertise’s adaptability opens the door for detecting different pathogens, increasing its potential to revolutionize infectious illness diagnostics worldwide.
Extra info:
George Adedokun et al, A handheld HIV detection platform utilizing paper-based pattern preparation and real-time isothermal amplification, Microsystems & Nanoengineering (2024). DOI: 10.1038/s41378-024-00822-1
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Reworking HIV analysis: A low-cost, point-of-care detection answer (2025, February 19)
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