Main analyses: c-Myc (A) and p-GR (B) nuclear presence exhibits unremarkable distinction between non-healing and healed sufferers. Consultant photographs of immunoperoxidase staining of the mobile Myc (c-Myc) and phosphorylated glucocorticoid receptor (p-GR) of diabetic foot ulcer tissue are proven. The numbers above the pictures point out % of optimistic nuclear staining for every biomarker. Dashed line signifies the basement membrane. Scale bar = 200 μM. Boxplots present related distributions of baseline c-Myc and p-GR quantifications between healed and non-healed DFUs at week 12. Median baseline c-Myc was numerically larger within the healed group whereas median baseline of p-GR was numerically larger in not-healed group. Boxplot symbols: Diamond = imply, center line = median, backside field line = first quartile (Q1), prime field line = third quartile (Q3), whiskers are 1.5*IQR from Q1 to Q3. Credit score: Wound Restore and Regeneration (2025). DOI: 10.1111/wrr.70044
Diabetic foot ulcers (DFUs) are a significant scientific problem, with excessive charges of morbidity, incapacity, amputations and mortality. Regardless of intensive analysis, efficient predictive biomarkers for DFUs therapeutic stay elusive.
A brand new examine led by the Nationwide Institute of Diabetes and Digestive and Kidney Ailments (NIDDK) Diabetic Foot Consortium (DFC) and printed in Wound Restore and Regeneration evaluated the potential of two biomarkers, c-myc and phosphorylated-glucocorticoid receptor (p-GR), in wound tissue to foretell therapeutic outcomes in DFUs.
The examine represents an necessary preliminary step in figuring out biomarkers that may predict scientific outcomes, monitor progress or predict recurrence. The analysis additionally revealed essential insights into the challenges confronted in managing this complicated medical situation.
Most significantly, the examine created a worthwhile useful resource of wound-related organic samples coupled with complete scientific information to assist ongoing and future research aimed toward figuring out new DFU biomarkers.
The examine evaluated wound tissue collected at baseline from 107 individuals with energetic DFUs and assessed the nuclear presence of c-myc and phosphorylated-glucocorticoid receptor (p-GR) by way of immunohistochemistry. Whereas imply biomarker ranges have been related between ulcers that healed and people who continued, p-GR ranges aligned with the examine’s speculation, suggesting avenues for additional investigation.
The scientific examine was the DFC’s first and efficiently demonstrated the feasibility of integrating tissue biomarker evaluation into potential DFU analysis. The trouble resulted in a singular, well-characterized biorepository of wound tissue samples linked to scientific outcomes, which can function a essential platform for future discovery and validation of predictive markers.
“This study lays the groundwork for further biomarker discovery and advancing DFU science and clinical practice,” mentioned the College of Miami Miller Faculty of Medication’s Marjana Tomic-Canic, Ph.D., Professor of Dermatology on the Dr. Phillip Frost Division of Dermatology and Cutaneous Surgical procedure, Director of the Wound Therapeutic and Regenerative Medication Analysis Program and the lead investigator of the DFC’s College of Miami Biomarker Analyses Unit.
“Although there is still work to be done to develop biomarkers that are strong predictors of healing, the established infrastructure of the DFC is leading the way and will be crucial for supporting research focused on DFU healing.”
The DFC’s ongoing efforts to broaden this biorepository, mixed with superior applied sciences like spatial transcriptomics and proteomics, provide new alternatives to uncover novel biomarkers and enhance DFU administration. The examine reinforces the DFC’s dedication to advancing DFU science with its grasp protocol, “No DFU Patient Goes Unstudied,” making certain the analysis is generalizable to all people burdened by DFUs.
“This effort represents a critical resource for continued biomarker exploration. We are now in a stronger position to further investigate other molecules and technologies that may lead to reliable, predictive tests for DFU healing,” mentioned Dr. Tomic-Canic. “This study is just the beginning, and the resources generated will continue to drive the advancement of DFU research for years to come.”
Extra data:
Robert S. Kirsner et al, Analysis of c‐Myc and Phosphorylated Glucocorticoid Receptor (p‐GR) for Predicting Diabetic Foot Ulcer Therapeutic—A Diabetic Foot Consortium Research, Wound Restore and Regeneration (2025). DOI: 10.1111/wrr.70044
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Two biomarkers that will predict diabetic foot ulcer therapeutic outcomes recognized (2025, June 13)
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